留学生生物化学专业课程作业Experimental部分范文

留学生生物化学专业课程作业Experimental部分范文

来源:www.51fabiao.org作者:cinq发布时间:2013-09-29 14:19
这是一篇国外大学生物化学专业课程论文Experimental实验部分操作写作范文,供参考。

这是一篇国外大学生物化学专业课程论文Experimental实验部分操作写作范文,供参考。

Experimental
Standard preparation
Weigh accurately creatintine 79.17mg and then dissolve to the deionized water. And dilute with water to 100mL.7000 creatintine standard preparation succefully prepare.
Weigh accurately HA 30mg HA and then dilute with water to 100ml.

Calculation of sample dilution
Respectively measure 5ml Creatintine and HA standard prepareation, and mix. Finally, dilute with creatinine to 3500、1750、 875、 432.5、216.5、108.125、54.0625、 27.031 umol/L; Dilute with HA to 3000、1500、 750、 162.5、81.25、40.625、 20.312 umol/L
Method
Weigh 100ul urine, 3000r/min centrifuge for 10 min, and then get supernatant.  CE separations were carried out on a Waters Quanta 4000 CE system using a hydrostatic injection mode (10 cm elevation of sample) for 20 s, a positive power supply, and a detector wavelength of214 nm. The capillary was either 60 cm×75um or 45cm×75um (Polymicro Technologies, Phoenix, AZ, USA) with a 1.5 mm detector window 7.5 cm from one end. The data was collected using Lab Calc software from Galactic (Salem, NH, USA) with 1 V equal to 1 AU. Rinse times were 30 s 0.1 M sodium hydroxide, 30 s water, and 1 min buffer, using a vacuum pressure of 15 in.Hg (1in.Hg53386.38pa). Make the standard curve. Take the peak area as abscissa, and take the concentration as ordinate.